One of the blogs of the European Geosciences Union (EGU) has a nice feature about field rucksacks. Scientists reveal what they pack into their rucksack when they departure for field work. I have already introduced you to my field work at cockchafer infested sites in Germany, and now you will also get a chance to peek into my field rucksack.
But before I can unpack my field rucksack at a measurement site, I first have to get there. What I really like about Germany is the fact that one can reach almost any location by public transport. I usually take the train and for covering the distance between a train station and a field site, I have my bicycle. The bicycle is also very useful to carry the spade used for soil excavations.
And here is a list of all the items you can find when unpacking my field rucksack.
- A small suitcase with audio equipment (microphones and an external amplifier) for spying on cockchafer larvae in the soil.
- A folder with data sheets (one for each excavated soil plot).
- 35 glass test tubes (110 ml volume each) with rubber stoppers for a) incubation of larvae to quantify their methane emissions, and b) transport of bulk soil samples.
- A suitcase containing a sensor and data logger for measuring soil moisture and soil temperature at different soil depths. The suitcase can also be used as a small table.
- Stainless steel cylinders with caps to collect undisturbed soil samples. Undisturbed soil samples are necessary to quantify dry bulk density and gravimetric soil moisture content.
- A knife.
- A small scale to weigh excavated cockchafer larvae.
- A plastic box with useful small items (pens, batteries, hollow needles for syringes, ect.).
- A stop watch to time larval incubations in the glass test tubes.
- The other half of my audio equipment – headphones and an audio recorder.
- A water and dirt proof field book for taking notes in addition to what is recorded on the data sheets.
- A small shovel.
- A spoon (which I actually never used…)
- A box containing evacuated glass vials for gas sample collection and storage, and two plastic syringes (one spare) for gas sampling.
- A mobile, handheld weather station for measuring air temperature, air pressure, relative air humidity, and wind speed. A GPS device to determine the exact position of each dug up soil plot.
- From left to right: a tool to insert stainless steel cylinders into soil without disturbing it, a knife to remove protruding soil from the cylinders after sampling, a hammer to drive the steel cylinder tool into the soil, a folding yardstick, and wooden sticks to mark the plots for excavation.
- Sunscreen, insect repellent, disposable gloves (cockchafer larvae are not “house-trained”), plastic bags for bulk soil samples and trash, and tape.
For those interested in soil fauna, soil fungi and greenhouse gas fluxes, join us at the EGU in Vienna next year! We are looking forward to your contributions!
Detailed session info.
In the basement of our research institute, I keep a few living common cockchafer larvae which we had dug out in a pasture in Blaubeuren-Weiler (Germany) in May. The original plan was to use these larvae in an acoustic experiment to learn more about the communication between them. Well as it turned out, the larvae didn’t care about my schedule for the experiment. They had already reached their third and final larval stage when we collected them in the field. This means that in August/September, the larvae should start to pupate to eventually transform into the adult beetles. When food is abundant and soil temperatures are sufficiently warm, larvae can develop much faster and start to pupate earlier. It seems that my common cockchafers feel very much at home in the basement of the institute because that is exactly what happened. The majority of the larvae has already turned into pupae.
A healthy well-fed cockchafer larva is always clean (despite of living in soil), shiny and has a dark butt. The dark colour stems from soil and organic material in the larval gut. You can see its content shimmer through the skin which is transparent at this part of the larval body.
Common cockchafer larva – third larval stage (= third instar)
When the larva starts to pupate, it becomes inactive, stiff and starts to look like a little mummy. The skin becomes very dry and people who are unfamiliar with these animals might just think that the larva is dead.
Common cockchafer larva starting to pupate
To finally change from the larva into a pupa, the animal has to completely shed its skin. In the last picture, you see the final result – a common cockchafer pupa. The animal doesn’t look like an adult beetle (= imago) yet, but already quite different from the original larva. At the bottom of the picture, you can see the shed skin. If you look close enough you can still recognize the shape of the larval head.
Common cockchafer pupa
In our recent publication about the rose chafer – another scarab beetle – we could show that these insects stop emitting methane when they transform from the larva to the pupa. This makes sense because at this stage the insects completely stop feeding. And thus the methane-producing microorganisms living in the gut system of these insects eventually run out of food and their productivity goes down as well.
One objective of my research project „CH4ScarabDetect“ is to quantify directly in the field methane (CH4) emissions of cockchafer larvae at cockchafer infested sites. In May, I visited the Kaiserstuhl – a region in southwest Germany famous for its wine production. To the dismay of the wine-growers, cockchafer larvae like to feed on vine, but I didn’t plan to visit any vineyards that day.
When I started my journey in the morning, I thought I would spend my day amongst walnut trees in a tree nursery in a small town called Sasbach-Leiselheim. There is a family-owned tree nursery and the family Schott already has to deal with the common cockchafer (Melolontha melolontha) as a pest insect for over 30 years. The common cockchafer is one of the main pest insects at the Kaiserstuhl.
To my surprise, Mr. Schott senior drove me to a small Christmas tree plantation near Burkheim which belongs to another landowner. Christmas trees were never on my list of vegetation types I wanted to visit during my research project, but if the cockchafers like them, I like them as well.
Christmas trees which have been damaged by cockchafers.
For my soil excavations at cockchafer infested sites, I start with marking 50 cm x 50 cm large measurement plots with the help of a wooden frame. Then, I dig up the soil in these plots to a depth of about 25 – 30 cm. For each cockchafer larva that I find during digging I write down at which depth I found it and I measure its weight. At the Christmas tree plantation, I found up to 25 larvae per m² at different stages of larval development (weights ranging between 0.6 and 2.6 g). A fully-grown adult beetle was also among my findings.
Excavated measurement plot (50 cm x 50 cm, 25 cm deep).
A larva of the Common Cockchafer right below the soil surface.
To determine the CH4 emissions of the excavated larvae, each individual is placed inside a large glass test tube which is then sealed air-tight for an hour. We refer to this method as incubation and the time the test tube is closed as incubation time. During the incubation time, all CH4 which is emitted by the larva is collected in the test tube. After an hour, a small air sample (25 ml) is extracted from the test tube with a syringe. The air sample will be stored in a small glass vial and latter analysed with a gas chromatograph to determine its CH4 concentration. I don’t know yet how much CH4 the larvae emitted at the Christmas tree plantation on that day because the data of the gas chromatograph still has to be analyzed. However from other sites, I know that the CH4 concentration inside the glass test tubes can increase to more than 50 ppm within an hour which is far above the atmospheric concentration of CH4 in the air surrounding us, which is about 1.8 ppm.
Cockchafer larva in a glass test tube for a methane measurement.
This is the 6th time that I am attending the EGU and yesterday I managed for the first time to be busy the entire day with meetings and actually not listening to any presentation or looking at any poster. That’s what scientists normally do most of the time at conferences. Listening to other scientists presenting their studies. The EGU is thematically subdivided into 22 divisions. I am usually jumping around between sessions of Atmospheric Sciences, Biogeosciences and Soil System Sciences. This is enough to bring your calendar to explode because normally one ends up with at least three different sessions running at the same time, and of course, at opposite ends of a four-storey building. However at the EGU, it does not stop there. For me it is THE PLACE for networking and there are loads of additional sessions dealing exactly just with that.
First of all, there are so-called non-public splinter meetings. You want to sit down with scientists from other universities and work on something? You can book a meeting room at the EGU. Yesterday morning we were seven people sitting together for one and a half hour working on drafts for scientific papers from a measurement campaign. These meetings can also be organized as public meetings for networking in the scientific community (e.g. “Ideas and perspectives for future research on forests and the CH4 and N2O cycles” organized by Mari Pihlatie from Helsinki) and it is also possible to contribute actively to shaping the programme of the EGU General Assembly in 2018 by joining the subdivision meetings (e.g. Subdivision SSS4: Soil Biology, Microbiology and Biodiversity). If you are a young scientist, go to these meetings! It is a great way to increase your visibility in the research community. In addition to the scientific and administrative sessions and meetings, there is also a huge exhibition with companies presenting their scientific instruments. These companies are keen on getting to know our research interests and needs to design new product lines. In the best case, it is a win-win situation for both sides. I had a meeting with a LI-COR Biosciences representative to discuss what I like about the currently availabe suit of gas analyzers and what improvements and new developments I am looking for to use in future measurement campaigns. And of course at the end of the day, I had to present my poster.
Poster presentations at the EGU can be a bit daunting and overwhelming for both the presenters and the audience. There are huge halls where you have nothing but posters. “That poster abstract sounds interesting, but do I want to walk another kilometer today to find it? Brain, are you still capable of taking in new information?”.
And thus it can happen that you have invested a lot of time in preparing a nice poster and you are at a conference with over 10.000 scientists, but maybe only two or three actually stop at your poster and maybe one asks you a question. Yesterday, I had the best poster session at the EGU ever! I love my research topic and think it is absolutely awesome and cool. What a feeling when other people come to you and have the same opinion. As it turned out, my boss did a great job in advertising my poster during her talk in the morning. A lot of people came and said “I heard this talk in the morning, can you explain me more about this and that”. One and a half hours of author’s attendance time (the time you actually should stand next to your poster to answer questions) were just flying by and I stopped counting to how many people I spoke during that time. Yes, fresh motivational boost for the upcoming work!
To finish the day, I went to listening to a talk by Pete Smith. For all of you soil scientists out there, yes, THE Pete Smith. Yesterday, he received the Philippe Duchaufour Medal for his outstanding contribution to the field of soil science and this guy is just an awesome presenter. The title of his lecture might give a hint (“Soil science is way more fun than a proper job”). You should really have a look at his abstract. And don’t get back to the first sentence of this blog post. This didn’t count as listening to a presentation. That was relaxing after work.
Spring marks the beginning of a new field season. When I spent my days in the field measuring CH₄ fluxes between soils and the atmosphere, I am mostly all by myself. Last Thursday at my mesocosm experiment that little insect kept me company which didn’t mind being carried around with the measurement equipment.
However right now, I am in Vienna to visit one of the biggest conferences I can possibly attend in my reasearch area. The European Geosciences Union General Assembly 2017 has begun.
A full week of listening, watching, discussions, networking … over 900 sessions and more than 10000 attendees. Topics ranging literally from the Earth’s core all the way into outer space. You can even watch part of the action from home. For me, it will be the first time to present my project CH4ScarabDetect to a really large and diverse audience. If you are actually at the conference this week and interested to learn more about my project or insect CH₄ emissions in general, you can find me in poster hall X1 at board 17:30-19:00. My project supervisor, Prof. Claudia Kammann, is going to talk about rose chafers (Cetonia aurata) early in the morning in session SSS4.7.
EGU2017-12499 | Posters | SSS4.7
The inﬂuence of cockchafer larvae on net soil methane ﬂuxes under diﬀerent vegetation types – a mesocosm study
Carolyn-Monika Görres, Claudia Kammann, David Chesmore, and Christoph Müller
Tue, 25 Apr, 17:30–19:00, Hall X1, X1.221
EGU2017-12198 | Orals | SSS4.7
Stimulation of methane oxidation by CH4-emitting rose chafer larvae in well-aerated grassland soil
Claudia Kammann, Carolyn-Monika Görres, Stefan Ratering, and Christoph Mueller
Tue, 25 Apr, 08:45–09:00, Room -2.32
I study greenhouse gas fluxes between soils and the atmosphere, but I will never see my study object. I can hold a beetle larva in my hand knowing that it potentially can emit methane, but the gases stay invisible. I only ever see them indirectly as numbers on a screen or a printout. Thus it is essential for my work to be able to trust the output of analyzers, but also to trust data and publications made available by other scientists. At the same time, I have to be very critical about my own work and the work of others. All measurements have uncertainties and are prone to errors. Measurements can be interpreted in different ways. The combination of trust, critical assessment, sharing data and knowledge as well as open discussions are key elements of research integrity and the basis for advancing our knowledge. Alternative facts don’t help here. And that is why I marched for science today. And of course, to have a great time with a bunch of funny, cool and crazy people I have never met before.
Cockchafers belong to the family Scarabaeidae which includes more than 20000 other beetle species. Thus it is not unlikely that there are other beetles out there which also emit methane. We have just published a paper about the effect of the rose chafer (Cetonia aurata) on soil methane fluxes. Just follow the link below.
On 25th March 1957, 60 years ago, the Treaty of Rome was signed. That marked the beginning of what we know today as the European Union. Since then, the EU has funded many research projects, both in basic and applied research, e.g. through the Marie Sklodowska-Curie actions which fund my current project CH4ScarabDetect.
There is one thing that the EU does not want researchers to be – the infamous people in the ivory tower. The EU expects us to be mobile within Europe, to cooperate with research institutions in other countries, to connect to businesses and NGOs where applicable, and to communicate our research to the public and public stakeholders. How we accomplish this task is our own responsibility as researchers.
Today, I am in Salamanca at a conference of the Marie Curie Alumni Association. The goal of the Marie Curie Alumni Association is “promoting an active community of researchers benefiting from the European Commission’s Marie Curie programme”. Todays topics cover ‘Protecting Freedom in Science’, ‘Science and Business’, ‘International Mobility and and Mobility of Scientists’, and ‘How can MCAA help display researchers’. This conference is open for everyone and you are welcome to tune and listen to the keynote speeches in this livestream.
I am sure some of you have already seen may beetles flying around in springtime. But who of you has ever observed a may beetle larva digging in the soil? Probably not so many. Well here is your chance to change that. Just to get an impression of the size of the larva in the video, in the upper left corner you can see some carrots which are ready to be harvested.